YSI 9300 and 9500 Photometers User Manual ®
7 Set the dilution factor to ‘On’ or ‘Off’. If the dilution factor option is set to ‘On’, the instrument will allow the entry of a numerical fact
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Oxygen is a major
Test Procedure 1 Filter sample if necessary to obtain a clear solution. 2 Fill the test tube with sample to the 10 ml mark. 3 Add one Sulfitest N
PHOTOMETER TEST INSTRUCTIONS ________________________________________________________________________________________ Zinc compounds are
SEPARATION OF RESIDUALS The photometer is programmed for both zinc and the copper correction procedure. Use program Phot 35 Zinc (+ Co
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ The Alkalinity of
Reagents and Equipment YSI Alkaphot M Tablets YSI Alkaphot P Tablets YSI 9300 or 9500 Photometer Round Test Tubes, 10 ml glass (PT 595) Test Proc
NOTE The expression of alkalinity results sometimes causes confusion. It is normal practice to express the result as mg/l CaCO3 (calcium c
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Iron occurs wide
Reagents and Equipment YSI Iron MR No 1 Tablets YSI Iron MR No 2 Tablets YSI Citrate IR Tablets YSI 9300 or 9500 Photometer Round Test Tubes, 10 ml
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Hydrazine is used a
8 Units The photometer offers the choice of result expressed in mg/l, ppm, mmol/l, µmol and g/l. Sample Number (9500 Only) A unique number may be
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Formulations c
Test Procedure 1 Filter sample if necessary to obtain a clear solution. 2 Fill test tube with sample to the 10 ml mark. 3 Add one Molybdate No 1
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Nitrites and nitrite
Test Procedure 1 Filter sample if necessary to obtain a clear solution. 2 Using the measuring syringe take 1 ml of the sample. Transfer to the tes
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ The use of organop
Reagents and Equipment YSI Oxidising OP Tablets YSI OP-A Tablets YSI OP-B Tablets YSI OP-AX Tablets YSI 9300 or 9500 Photometer YSI Test Tube, 20 ml
Test Procedure - Correction Factor If it is suspected that the sample contains orthophosphate, carry out the following correction procedu
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ The YSI Chloridol te
Reagents and Equipment YSI Acidifying CD Tablets YSI Chloridol Tablets YSI 9300 or 9500 Photometer Round Test Tubes,
5 Select the appropriate program number on the photometer for the test range required. 6 Take the photometer reading in usual manner (see photomet
9 Locking System Mode Settings (9500 Only) It is possible to 'lock’ the system settings so that these cannot be tampered with or altered a
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Pure water exhibits
Test Procedure 1 Filter sample through a GF/B filter paper. 2 Fill a test tube with filtered sample to the 10 ml mark. 3 Fill a test tube with de
PHOTOMETER TEST INSTRUCTIONS ________________________________________________________________________________________ Turbidity is an impo
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ The presence of d
Test Procedure Read the Oxygen test instructions leaflet contained in the CHEMetrics Vacu-Vials pack. Observe these various recommendations rega
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ DISSOLVED OXYGEN/
Test Procedure Read the Oxygen test instructions leaflet contained in the CHEMetrics Vacu-Vials pack. Observe these various recommendations rega
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ PHMB Polyhexamethylb
Test Procedure 1 Fill the test tube with sample to the 10 ml mark. 2 Add one PHMB-Phot tablet crush and mix to dissolve. 3 Select Phot 52 on phot
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Nickel does not occ
10 USB (9500 Only) The USB interface allows communication between the instrument and a PC. There is a choice of two operating modes – Hard Drive an
Test Procedure 1 Fill test tube with sample to the 10 ml mark. 2 Add one Nickeltest No 1 tablet, crush and mix to dissolve. Ensure tablet is compl
PHOTOMETER TEST INSTRUCTIONS ___________________________________________________________________________________________ Phenols and substi
Reagents and Equipment YSI Phenoltest No 1 Tablets YSI Phenoltest No 2 Tablets YSI Phenoltest PR Tablets YSI 9300 or 9500 Photometer Round Test
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Chromium may be pre
Reagents and Equipment For Hexavalent Chromium :- YSI Chromicol No 1 Tablets YSI Chromicol No 2 Tablets YSI 9300 or 9500 Photometer YSI Test Tube
Test Procedure - Total Chromium 1 Fill the pretreatment tube with sample to the 20 ml mark. 2 Add one level spoonful of Chromicol CR powder
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Silicon, in the
Test Procedure 1 Fill the test tube with sample to the 10 ml mark. 2 Add one Silica No 1 tablet, crush and mix to dissolve. 3 Add one Silica No 2
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Chlorine dioxide i
Important Note Chlorine dioxide is extremely volatile and can be lost from solution very easily. Extreme care must be taken when extracting and disp
11 4 INTERFACE CONNECTIONS AND DATA MEMORY (9500 ONLY) Stored data can be accessed by recall to the instrument display (see ‘View Log’). A
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Chlorine dioxide i
Important Note Chlorine dioxide is extremely volatile and can be lost from solution very easily. Extreme care must be taken when extracting and disp
Item # Y-PT 282 Rev A Drawing # Y-PT 282 © 2010 YSI IncorporatedV1-11/10
12 5 TAKING PHOTOMETER READINGS The photometer is very simple to use. Screen prompts guide the user towards the test result. The follo
13 The BLANK TUBE is a test tube filled only with the water being tested only. It is important to use the actual water to be tested to provide a tru
14 If the sample number option is pre-selected, then the following display will appear, for example (9500 only): Enter or confirm the sample num
15 3 The instrument will be set automatically. After a few seconds the following display will appear: Place SAMPLE TUBE in the test chamber
16 Continuation Tests (Certain Tests Only) 1 Select ‘Follow-On’ and press [OK] while the result is displayed of the currently running
YSI 9300 and 9500 Direct-Read Photometers User Manual YSI, Inc. 1725 Brannum Lane Yellow Springs, OH 45387 Tel: 800-897-4151 (+1 937-767-72
17 Reading in Transmittance and Absorbance When taking readings in Transmittance or Absorbance mode, use the and keys to step through the
18 To program user-defined calibrations: Turn the instrument ‘ON’, select ‘System’ menu and press [OK]. Scroll through the options to the USB entry
19 An example is shown below: USER CALIBRATION 900 Chlorine mmol/l 500nm Cl2 0,0 0.174,0.50 0.481,1.50 0.733,2.50 0.854,3.00 1.022,4.00 1.086,4.50 1
20 6 CARE AND MAINTENANCE The photometer is designed to give long and trouble-free operation. Care must be taken, however, to avoid
21 Photometer Up-Grade (9500 only) It is now possible to upgrade the photometer with new test calibrations using a computer. This will ensure that u
22 Limitation of Warranty This Warranty does not apply to any YSI product damage or failure caused by: 1 Failure to install, operate or use
Table of Contents Photometer Instructions PHOT.INST. TABLET REAGENT SYSTEM Alkalinity, Total (Alkaphot)
Direct-Reading Photometer Program Schedule TABLET REAGENT SYSTEM Instruction Sheet Number Reagent System Parameter Photometer Program Number - - Tr
Instruction Sheet Number Reagent System Parameter Photometer Program Number PHOT.23. Nitrate (Nitratest) Nitrate Nitrogen Phot 23 PHOT.24. Nitrite
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Natural and treat
INDEX 1 INTRODUCTION ... 1 Fe
Test Procedure 1 Fill test tube with sample to the 10 ml mark. 2 Add one Alkaphot tablet, crush and mix until all of the particles have dissolved.
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Aluminum sulphate is widel
Sample Collection Aluminum is readily absorbed on to the surfaces of sample containers, particularly glass containers. To avoid loss of aluminum, co
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Ammonia occurs as
Test Instructions 1 Fill test tube with sample to the 10 ml mark. 2 Add one Ammonia No 1 tablet and one Ammonia No 2 tablet, crush and mix to dis
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Bromine and brom
Reagents and Equipment YSI DPD No 1 Clear Tablets YSI DPD Nitrite Tablets YSI 9
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Chlorine and chlor
Reagents and Equipment YSI DPD No 1 Tablets YSI DPD No 2 Tablets YSI DPD No 3 Tablets YSI 9300 or 9500 Photometer Round Test Tu
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ CHLORINE DIOXIDE
1 1 INTRODUCTION The YSI 9300 and 9500 direct-read photometers are designed to give long and trouble-free operation. To ensure the best resul
Reagents and Equipment YSI DPD No 1 Tablets YSI DPD No 3 Tablets YSI DPD Glycine Tablets YSI DPD Acidifying Tablets YSI DPD Neutralising Tablets YSI
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Chlorine dioxide is
Reagents and Equipment YSI DPD No 1 Tablets YSI DPD No 3 Tablets YSI DPD Glycine Tablets YSI DPD Acidifying Tablets YSI DPD Neutralizing Tables YSI
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Chlorine and chlori
Reagents and Equipment YSI DPD No 1 Tablets YSI DPD Oxystop Tablets (Optional) YSI DPD No 3 Tablets YSI 9300 or 9500 Photometer Round Test Tubes,
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Chlorine and chlorine relea
Reagents and Equipment YSI DPD No 1 Tablets YSI DPD No 2 Tablets YSI DPD No 3 Tablets YSI 9300 or 9500 Photometer Round Test Tu
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ CHLORINE DIOXIDE
Reagents and Equipment YSI DPD No 1 Tablets YSI DPD No 3 Tablets YSI DPD Glycine Tablets YSI DPD Acidifying Tablets YSI DPD Neutralising Tablets YSI
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Chlorine dioxide i
2 Instrument Layout Test and Number Keys Multi-Size Cell Holder OK (Enter) Key Scroll and Selection Keys On/Off Key USB Interfac
Reagents and Equipment YSI DPD No 1 Tablets YSI DPD No 3 Tablets YSI DPD Glycine Tablets YSI DPD Acidifying Tablets YSI DPD Neutralizing Tables YSI
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Chlorine and chlorin
Reagents and Equipment YSI DPD No 1 Tablets YSI DPD Oxystop Tablets (Optional) YSI DPD No 3 Tablets YSI 9300 or 9500 Photometer Round Test Tubes,
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Chlorine and chlor
Test Instructions 1 Rinse test tube with sample leaving two or three drops of sample in the tube. 2 Add one DPD No 4 tablet, crush tablet and then
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Chlorine and chlo
Test Procedure 1 Fill test tube with sample to the 10 ml mark. 2 Add one Acidifying GP tablet and one Chlorine HR tablet. Crush tablets and mix to
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Copper occurs natu
Separation of Copper Residuals The direct-reading photometer is programmed for both free and total copper. Use program Phot 10 Free Copper, then
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Calcium hardness
3 Features and Technical Specification Application For application in general water testing using Palintest tablet reagent systems and Palin
Test Procedure 1 Filter sample if necessary to obtain a clear solution. 2 Fill the test tube with sample to the 10 ml mark. 3 Add one Calcicol No
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Cyanuric acid is
Test Procedure 1 Fill test tube with sample to the 10 ml mark. 2 Add one Cyanuric Acid tablet and allow to disintegrate for at least t
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Fluoride occurs n
TEST PROCEDURE 1 Fill test tube with sample to the 10 ml mark. 2 Add one Fluoride No 1 tablet, crush and mix to dissolve. 3 Add one Fluoride No 2
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Water hardness i
Test Procedure 1 Filter sample if necessary to obtain a clear solution. 2 Fill test tube with sample to the 10 ml mark. 3 Add one Hardicol No 1 t
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Hydrogen peroxide is
Test Procedure 1 Rinse test tube with sample leaving 2 to 3 drops of sample in the tube. 2 Add one Hydrogen Peroxide LR tablet, crush and then fi
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Hydrogen peroxide
4 2 OPERATING PRINCIPLE The YSI photometers are instruments that measure color intensity. Light is passed through a test tube containing
Test Procedure 1 Fill test tube with sample to the 10 ml mark. 2 Add one Acidifying PT tablet and one Hydrogen Peroxide HR tablet. Crush tablets
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Iron occurs widely
Reagents and Equipment YSI Iron LR Tablets YSI 9300 or 9500 Photometer Round Test Tubes 10 ml glass (PT 595) Test Procedure 1 Fill the test tube w
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Iron occurs widely i
Test Procedure 1 Fill test tube with sample to the 10 ml mark. 2 Add one Iron HR tablet, crush and mix to dissolve. 3 Stand for one minute to all
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Manganese-containi
Reagents and Equipment YSI Manganese No 1 Tablets YSI Manganese No 2 Tablets YSI 9300 or 9500 Photometer Round Test Tubes, 10 ml glass (PT 595)
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Magnesium is a
TEST PROCEDURE 1 Using the measuring syringe take a 1 ml sample of the water under test. Transfer to the round test tube and make up to t
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ MOLYBDATE HR Formu
5 Replacing the Batteries The battery compartment in the base of the instrument is secured by four screws. To replace the batteries, remove the cove
Reagents and Equipment YSI Molybdate No 1 HR Tablets YSI Molybdate No 2 HR Tablets YSI 9300 or 9500 Photometer Round Test Tubes, 10 ml glass (PT
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Nitrates are norm
Reagents and Equipment YSI Nitratest Powder (Spoon Pack) YSI Nitratest Tablets YSI Nitricol Tablets YSI Nitr
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Nitrites are foun
Test Procedure 1 Fill round test tube with sample to the 10 ml mark. 2 Add one Nitricol tablet, crush and mix to dissolve. 3 Stand for 10 minutes
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Ozone is used for
Reagents and Equipment YSI DPD No 4 Clear Tablets YSI DPD Glycine Tablets YSI 9300 or 9500 Photometer Round Test Tubes, 10 ml glass (PT 595) Se
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ pH measurement i
Notes 1 The color range of the phenol red test is yellow, through orange, to red. The formation of an intense purple coloration shows that the indi
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Phosphates are ex
6 3 GENERAL PHOTOMETER OPERATION The photometer is controlled by a simple intuitive menu system: The highlight indicates the active line or se
Reagents and Equipment YSI Phosphate No 1 LR Tablets YSI Phosphate No 2 LR Tablets YSI 9300 or 9500 Photometer Round Test Tubes, 10 ml glass (PT
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Phosphates are ext
Test Procedure 1 Fill test tube with sample to the 10 ml mark. IN THE CASE OF SAMPLES CONTAINING SILICA (>20 mg/l SiO2) ONLY :- 2 Add one Phosp
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Potassium is an abu
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Silicon, in the
Test Instructions 1 Fill test tube with sample to the 10 ml mark 2 Add one Silica No 1 tablet, crush and mix to dissolve. Stand for five minutes t
PHOTOMETER TEST INSTRUCTIONS __________________________________________________________________________________________ Sulfates occur natu
Reagents and Equipment YSI Sulfate Turb Tablets YSI 9300 or 9500 Photometer Round Test Tubes, 10 ml glass (PT 595) Test Procedure 1 Fill test tube
PHOTOMETER TEST INSTRUCTIONS _________________________________________________________________________________________ Natural waters co
Sample Collection To prevent loss of sulphide collect the sample carefully with a minimum of agitation or aeration. Test the sample as s
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